![]() Gating strategy for extracellular staining for CD4 +, CD8α +, CD4 +CD8α + and IgM + cells in spleen sample of the examined turkeys. ![]() This image is from an open access article distributed under the terms of the Creative Commons Attribution License.įITC conjugated Goat anti Chicken IgM antibody ( AAI27F) used to label turkey lymphocytes by flow cytometry. ![]() The impact of Aloe vera and licorice extracts on selected mechanisms of humoral and cell-mediated immunity in pigeons experimentally infected with PPMV-1. Percentage of IgM + B cells at 4, 7 and 14 dpi in healthy pigeons administered herbal extracts (left) and in pigeons administered herbal extracts and inoculated with PPMV-1 (right)įrom: Dziewulska D, Stenzel T, Śmiałek M, Tykałowski B, Koncicki A. An asterisk indicates statistically significant differences (α<0.05) between groups.įITC conjugated Goat anti Chicken IgM antibody (AAI27F) used to evaluate of the number of IgM expressing B cells on Pigeon mononuclear cells by flow cytometry. The amount of antigen-specific antibodies was expressed in a relation to the reference sera (S/R, sample to reference ratio). Graphs represent the analysis of individual animals, displayed as a box-and-whisker plot. Serum IgY (A, B), IgM (C, D) and IgA (E, F) antibody responses are indicated. Kinetics of NHC-specific antibody levels following in ovo vaccination.ĮLISA results demonstrating the NHC flagellin-specific reactivity of serially diluted sera collected from (A, C, E) 12 day-old and (B, D, F) 18 day-old chickens injected with 40 μg of NHC flagellin or solvent (control). PLoS ONE 11(10): e0164837.This is from an open access article distributed under the terms of the Creative Commons Attribution License. An asterisk indicates statistically significant differences (α<0.05) between compared groups.įrom: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)Ĭhicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni. Results are displayed as a box-and-whisker plot (S/R, sample to reference ratio). Antigen-specific IgY, IgM or IgA antibody levels were determined in 20-fold diluted sera collected from 12 day-old individual animals. NHC flagellin-specific antibody levels in the groups immunized with (A) 40 μg of NHC protein and (B) 20 μg of NHC protein, both compared to levels in the solvent injected control group. Alexa Fluor® 647 conjugates are less expensive alternatives to allophycocyanin conjugates for flow cytometry.Horseradish peroxidase conjugated Goat anti Chicken IgM antibody ( AAI27P) used for the evaluation of flagellin specific IgM response in exposed chickens by ELISA.Īntigen-specific serum antibody responses. ![]() It is most commonly visualized with a confocal microscope equipped with an appropriate laser for excitation and a far-red detector. Therefore, Alexa Fluor® 647 is not recommended for use with conventional epifluorescent microscopes. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. Alexa Fluor® 647 conjugates are the best choice of far red-emitting dyes for multiple-labeling detection with a confocal microscope.Ī significant advantage of using Alexa Fluor® 647 over lower wavelength-emitting dyes is the low autofluorescence of biological specimens in this region of the spectrum. Alexa Fluor® 647- and APC-conjugated secondary antibodies are the best choice for flow cytometry when secondary antibodies fluorescing at these wavelengths are desired. They are brighter than Cy5 and DyLight 650 in aqueous mounting media. Alexa Fluor® 647-conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm. ![]()
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